António Lobo Antunes, Portuguese novelist who chronicled dictatorship and war, dies aged 83

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Greater Than (0): Everything in this space must be greater than 0. The answer is 2-3, placed horizontally.

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The next year, PacBio developed a new method to allow multiple sequencing passes on the same DNA molecule. Double stranded DNA templates were ligated to two single stranded adapters, creating what the company called a “SMRTbell template.” Sequencing began at a primer on one of the adaptors and could proceed multiple times per molecule due to circularization, in a process called rolling-circle amplification. This helped reduce PacBio’s error rates significantly.

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