This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
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1983 to 1988, making up almost a decade of IBM's efforts and very few sales.
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Fanny Angelina (née Eilshemius, from a Dutch father) grew up in Kearny, New Jersey. During her childhood, her family learned from a Dutch friend or neighbor about agar-agar, a common ingredient in jelly desserts in Java (Indonesia), then a Dutch colony. Her mother and, later, Fanny Angelina herself, began to cook with it.
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